Immunoenzyme kuongororwa kweropa - chidzidzo chekutsanangurira huwandu hwehuwandu hwehutano hwehutano hwemaantijeni nemasimba. ELISA inzira inoshandiswa mune zvakasiyana-siyana zvehutano, asi kazhinji inorwara zvirwere zvinotapukira, somuenzaniso, HIV , hepatitis, herpes uye zvirwere zvepabonde.
Nheyo yekuita imwe enzyme kukura
Immunoenzyme kuongorora ropa nokuda kwechirwere chetachiona, kushandiswa kwezvirwere kana kuvapo kwezvipembenene zvinoitwa, nokuti ndiye iye anogadza chirwere chakakwana, pamwe nehutachiona hwemurwere. Iyi nzira inopa 90% zvakarurama.
Masoja evanhu, iyo inopinda mune imwe nyika antigen, inogadzira mapuroteni chaiwo anonzi antibodies kuti auraye chirwere chacho. Zvirwere zvinopesana, sezvakadaro, zvinosungira maantigen, nokudaro dzichiita zvakasiyana-siyana antigen / antibody complexes. Tsanangudzo yakajeka yekuongorora kwe-immune-enzyme yeropa inoratidza kuti zvakaoma sei izvi. Somuenzaniso, mumamiriro ezvinhu apo zvakakosha kuziva chirwere chimwechete muropa (kana, kuti rive rakanyatsojeka, antigen yake), an anti-antibody chaiyo kune hutachiwana anowedzera kwairi.
Tsanangudzo yekutsvaga migumisiro
Migumisiro ye enzyme immunoassay yakaratidza kuvapo kwe immunoglobulin G? Ichi ndicho chimiro, sezvo chiratidzo ichocho chinoreva kuti causative agent wechirwere chaicho chaicho chiri mumuviri, asi panguva imwechete ma antibodies kwaive atosimbiswa uye murwere haadi chirwere.
Muchiitiko chacho kana utachiona hwepakutanga, uye muropa remurwere mushure meine enzyme immunoassay yekutengesa kana zvimwe zvirwere, immunoglobulins yekirasi M inoonekwa, matanho ekurapa anofanira kuitwa zvichidikanwa. Asi kana zvizoitika zveizvi kuongororwa zvakasimbisa kuvapo kwemasikirwo emakirasi M uye G, izvi zvinoratidza kuti chirwere chacho chave chiri mumatanho akaoma uye murwere anoda chirwere chekurumidza.
Kubatsira kwe enzyme immunoassay
Zvinobatsira zve enzyme immunoassay yezvipembenene, HIV, venereal uye zvirwere zvepachivande nezvimwe zvirwere ndeyokuti nzira iyi yekuongorora:
- icharatidza mhedzisiro chaiye muzvikamu zvekutanga zvechirwere;
- inototanga kuziva makirasi e-immunoglobulins;
- icharatidza simba rehutachiona;
- inopa mhinduro nokukurumidza;
- zvakanakisisa zvekupfuura;
- inokosha kukosha.
Chinhu chimwe chete chinokonzerwa nekuongororwa uku ndechokuti mune dzimwe nguva iyo ELISA inobereka manyepo-asina kunaka kana nhema-akanaka. Ndicho chikonzero kududzirwa kwemigumisiro kunofanira kushandiswa chete nemunhu anoziva zvakakwana.